SATB1 Mouse Monoclonal Antibody [Clone ID: OTI3E12]

CAT#: CF500578

Carrier-free (BSA/glycerol-free) SATB1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1. Positive lysates LY418976 (100ug) and LC418976 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500578)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500578)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500578)

Specifications

Product Data

• Clone Name: OTI3E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SATB1 (NP_002962) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.8 kDa
• Gene Name: Homo sapiens SATB homeobox 1 (SATB1), transcript variant 1, mRNA.
• Database Link:
  NP_002962
  Entrez Gene 20230 MouseEntrez Gene 316164 RatEntrez Gene 6304 Human
  UniProt ID Q01826
• Background: This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: DNA-binding protein SATB1; SATB homeobox 1; scaffold-associating DNA's); special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix

Reference Data

• Protein Families: Druggable Genome, Transcription Factors