XRCC1 Mouse Monoclonal Antibody [Clone ID: OTI2D8]

CAT#: CF500886

Carrier-free (BSA/glycerol-free) XRCC1 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1 (1:1000).

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-XRCC1 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500886)

• HEK293T cells transfected with either RC204952 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XRCC1 antibody (TA500886), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-XRCC1 antibody (TA500886), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2D8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.3 kDa
• Gene Name: Homo sapiens X-ray repair cross complementing 1 (XRCC1), mRNA.
• Database Link:
  NP_006288
  Entrez Gene 22594 MouseEntrez Gene 84495 RatEntrez Gene 7515 Human
  UniProt ID P18887
• Background: The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]
• Synonyms: RCC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair