Transketolase (TKT) Mouse Monoclonal Antibody [Clone ID: OTI5H3]

CAT#: CF500892

Carrier-free (BSA/glycerol-free) TKT mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TKT (RC203497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TKT. Positive lysates LY400434 (100ug) and LC400434 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TKT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-TKT mouse monoclonal antibody (TA500892) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TKT (RC203497).

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  Flow cytometric analysis of Hela cells, using anti-TKT antibody (TA500892), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Jurkat cells, using anti-TKT antibody (TA500892), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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Specifications

Product Data

• Clone Name: OTI5H3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TKT (NP_001055) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.9 kDa
• Gene Name: Homo sapiens transketolase (TKT), transcript variant 1, mRNA.
• Database Link:
  NP_001055
  Entrez Gene 21881 MouseEntrez Gene 64524 RatEntrez Gene 7086 Human
  UniProt ID P29401
• Synonyms: HEL107; TK; TKT1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway