LIPG Mouse Monoclonal Antibody [Clone ID: OTI8E4]

CAT#: CF501048

Carrier-free (BSA/glycerol-free) LIPG mouse monoclonal antibody, clone OTI8E4 (formerly 8E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG. Positive lysates LY401821 (100ug) and LC401821 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501048)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501048)

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  Anti-LIPG mouse monoclonal antibody (TA501048) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG (RC209248).

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  Immunoprecipitation (IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LIPG antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC209248 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody (TA501048), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI8E4
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.6 kDa
• Gene Name: Homo sapiens lipase G, endothelial type (LIPG), transcript variant 1, mRNA.
• Database Link:
  NP_006024
  Entrez Gene 9388 Human
  UniProt ID Q9Y5X9
• Background: The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]
• Synonyms: EDL; EL; PRO719

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways