MTMR14 Mouse Monoclonal Antibody [Clone ID: OTI9G9]

CAT#: CF501712

Carrier-free (BSA/glycerol-free) MTMR14 mouse monoclonal antibody, clone OTI9G9 (formerly 9G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR14 (RC207732, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR14. Positive lysates LY421457 (100ug) and LC421457 (20ug) can be purchased separately from OriGene.

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  Anti-MTMR14 mouse monoclonal antibody (TA501712) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MTMR14 (RC207732).

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  HEK293T cells transfected with either RC207732 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MTMR14 antibody (TA501712), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI9G9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MTMR14 (NP_001070993) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.0 kDa
• Gene Name: Homo sapiens myotubularin related protein 14 (MTMR14), transcript variant 2, mRNA.
• Database Link:
  NP_001070993
  Entrez Gene 97287 MouseEntrez Gene 312634 RatEntrez Gene 64419 Human
  UniProt ID Q8NCE2
• Background: This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.
• Synonyms: C3orf29

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Secreted Protein