RBBP9 Mouse Monoclonal Antibody [Clone ID: OTI4E1]

CAT#: CF501740

Carrier-free (BSA/glycerol-free) RBBP9 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBBP9 (RC202090, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP9. Positive lysates LY401976 (100ug) and LC401976 (20ug) can be purchased separately from OriGene.

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  Anti-RBBP9 mouse monoclonal antibody (TA501740) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RBBP9 (RC202090).

Specifications

Product Data

• Clone Name: OTI4E1
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RBBP9 (NP_006597) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.8 kDa
• Gene Name: Homo sapiens RB binding protein 9, serine hydrolase (RBBP9), mRNA.
• Database Link:
  NP_006597
  Entrez Gene 10741 Human
  UniProt ID O75884
• Background: The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: BOG; RBBP10