FKBPL Mouse Monoclonal Antibody [Clone ID: OTI2D8]

CAT#: CF502165

Carrier-free (BSA/glycerol-free) FKBPL mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBPL (RC202153, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBPL. Positive lysates LY411782 (100ug) and LC411782 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502165)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FKBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502165)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FKBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502165)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FKBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502165)

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  Anti-FKBPL mouse monoclonal antibody (TA502165) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FKBPL (RC202153).

• Flow cytometric Analysis of Hela cells, using anti-FKBPL antibody (TA502165), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-FKBPL antibody (TA502165), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2D8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FKBPL (NP_071393) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: Homo sapiens FKBP prolyl isomerase like (FKBPL), mRNA.
• Database Link:
  NP_071393
  Entrez Gene 63943 Human
  UniProt ID Q9UIM3
• Background: The protein encoded by this gene has similarity to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The encoded protein is thought to have a potential role in the induced radioresistance. Also it appears to have some involvement in the control of the cell cycle. [provided by RefSeq, Jul 2008]
• Synonyms: DIR1; NG7; WISP39