Dystrobrevin alpha (DTNA) Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF502184

Carrier-free (BSA/glycerol-free) DTNA mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNA (RC223952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNA. Positive lysates LY409817 (100ug) and LC409817 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNA monoclonal antibody.

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  Anti-DTNA mouse monoclonal antibody (TA502184) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNA (RC223952).

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  HEK293T cells transfected with either RC223952 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DTNA antibody (TA502184), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DTNA (NP_116761) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.7 kDa
• Gene Name: Homo sapiens dystrobrevin alpha (DTNA), transcript variant 5, mRNA.
• Database Link:
  NP_116761
  Entrez Gene 1837 Human
  UniProt ID Q9Y4J8
• Background: The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1

Reference Data

• Protein Families: Druggable Genome