MMAB Mouse Monoclonal Antibody [Clone ID: OTI1G3]

CAT#: CF502265

Carrier-free (BSA/glycerol-free) MMAB mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (RC204290, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB. Positive lysates LY409455 (100ug) and LC409455 (20ug) can be purchased separately from OriGene.

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  Anti-MMAB mouse monoclonal antibody (TA502265) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMAB (RC204290).

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  HEK293T cells transfected with either RC204290 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody (TA502265), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MMAB (NP_443077) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24 kDa
• Gene Name: Homo sapiens metabolism of cobalamin associated B (MMAB), transcript variant 1, mRNA.
• Database Link:
  NP_443077
  Entrez Gene 326625 Human
  UniProt ID Q96EY8
• Background: This gene encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. [provided by RefSeq]
• Synonyms: ATR; cblB; CFAP23; cob

Reference Data

• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism