PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12]
CAT#: CF502437
Carrier-free (BSA/glycerol-free) PRKCE mouse monoclonal antibody, clone OTI2A12 (formerly 2A12)
Formulation: Standard
Specifications
Product Data | |
Clone Name | OTI2A12 |
Applications | FC, IF, IHC, WB |
Recommended Dilution | WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100 |
Reactivities | Human, Dog, Monkey, Mouse, Rat |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 83.5 kDa |
Gene Name | protein kinase C epsilon |
Database Link | |
Background | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq] |
Synonyms | nPKC-epsilon; PKCE |
Reference Data | |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Antibody Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TA502437 | PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2A12 (formerly 2A12) |
USD 379.00 |
|
TA502437S | PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2A12 (formerly 2A12) |
USD 159.00 |
|
TA700072 | PRKCE biotinylated detection antibody, ELISA and Luminex validated mouse monoclonal antibody, clone OTI2A12 |
USD 399.00 |
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