ASCC2 Mouse Monoclonal Antibody [Clone ID: OTI1E8]

CAT#: CF502806

Carrier-free (BSA/glycerol-free) ASCC2 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (RC203391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2. Positive lysates LY403150 (100ug) and LC403150 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ASCC2-Knockout HeLa cells (KO, Cat# LC833296) were separated by SDS-PAGE and immunoblotted with anti-ASCC2 monoclonal antibody TA502806 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Anti-ASCC2 mouse monoclonal antibody (TA502806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2 (RC203391 ).

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  Flow cytometric Analysis of Hela cells, using anti-ASCC2 antibody (TA502806), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1E8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ASCC2 (NP_115580) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.2 kDa
• Gene Name: Homo sapiens activating signal cointegrator 1 complex subunit 2 (ASCC2), transcript variant 1, mRNA.
• Database Link:
  NP_115580
  Entrez Gene 84164 Human
  UniProt ID Q9H1I8
• Synonyms: ASC1p100; p100

Reference Data

• Protein Families: Druggable Genome, Transcription Factors