Monoacylglycerol Lipase (MGLL) Mouse Monoclonal Antibody [Clone ID: OTI1H1]

CAT#: CF502932

Carrier-free (BSA/glycerol-free) MGLL mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGLL (RC218358, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGLL. Positive lysates LY402124 (100ug) and LC402124 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502932)

• Anti-MGLL mouse monoclonal antibody (TA502932) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MGLL (RC218358).

• Immunofluorescent staining of HeLa cells using anti-MGLL mouse monoclonal antibody (TA502932).

• HEK293T cells transfected with either RC218358 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MGLL antibody (TA502932), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-MGLL antibody (TA502932), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-MGLL antibody (TA502932), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1H1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MGLL (NP_009214) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.1 kDa
• Gene Name: Homo sapiens monoglyceride lipase (MGLL), transcript variant 1, mRNA.
• Database Link:
  NP_009214
  Entrez Gene 11343 Human
  UniProt ID Q99685
• Background: This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
• Synonyms: HU-K5; HUK5; MAGL; MGL

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways