C1orf50 Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: CF502937

Carrier-free (BSA/glycerol-free) C1orf50 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1orf50 (RC200134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1orf50. Positive lysates LY411368 (100ug) and LC411368 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C1orf50 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502937)

• Anti-C1orf50 mouse monoclonal antibody (TA502937) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C1orf50 (RC200134).

• HEK293T cells transfected with either RC200134 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C1orf50 antibody (TA502937), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human (NP_077002) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.7 kDa
• Gene Name: Homo sapiens chromosome 1 open reading frame 50 (C1orf50), transcript variant 1, mRNA.
• Database Link:
  NP_077002
  Entrez Gene 79078 Human
  UniProt ID Q9BV19
• Synonyms: MGC955