XPNPEP3 Mouse Monoclonal Antibody [Clone ID: OTI5G2]

CAT#: CF503304

Carrier-free (BSA/glycerol-free) XPNPEP3 mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP3 (RC200888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP3. Positive lysates LY402903 (100ug) and LC402903 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503304)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503304)

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  Anti-XPNPEP3 mouse monoclonal antibody (TA503304) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP3 (RC200888).

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  HEK293T cells transfected with either RC200888 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPNPEP3 antibody (TA503304), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5G2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human XPNPEP3(NP_071381) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.9 kDa
• Gene Name: Homo sapiens X-prolyl aminopeptidase 3 (XPNPEP3), transcript variant 1, mRNA.
• Database Link:
  NP_071381
  Entrez Gene 321003 MouseEntrez Gene 685823 RatEntrez Gene 481237 DogEntrez Gene 705778 MonkeyEntrez Gene 63929 Human
  UniProt ID Q9NQH7
• Background: The protein encoded by this gene belongs to the family of X-pro-aminopeptidases that utilize a metal cofactor, and remove the N-terminal amino acid from peptides with a proline residue in the penultimate position. This protein has been shown to localize to the mitochondria of renal cells, and have a role in ciliary function. Mutations in this gene are associated with nephronophthisis-like nephropathy-1. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene, however, expression of some of these isoforms in vivo is not known.
• Synonyms: APP3; ICP55; NPHPL1

Reference Data

• Protein Families: Druggable Genome, Protease