MTFMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: CF503571

Carrier-free (BSA/glycerol-free) MTFMT mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTFMT (RC207666, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTFMT. Positive lysates LY408317 (100ug) and LC408317 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 175-389 of human MTFMT(NP_640335) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.7 kDa
• Gene Name: Homo sapiens mitochondrial methionyl-tRNA formyltransferase (MTFMT), mRNA.
• Database Link:
  NP_640335
  Entrez Gene 123263 Human
  UniProt ID Q96DP5
• Background: The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq, Jun 2011]
• Synonyms: COXPD15; FMT1

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis, One carbon pool by folate