AIPL1 Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF503572

Carrier-free (BSA/glycerol-free) AIPL1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AIPL1 (RC204079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AIPL1. Positive lysates LY415353 (100ug) and LC415353 (20ug) can be purchased separately from OriGene.

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  Anti-AIPL1 mouse monoclonal antibody (TA503572) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AIPL1 (RC204079).

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  HEK293T cells transfected with either RC204079 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AIPL1 antibody (TA503572), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AIPL1(NP_055151) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.7 kDa
• Gene Name: Homo sapiens aryl hydrocarbon receptor interacting protein like 1 (AIPL1), transcript variant 1, mRNA.
• Database Link:
  NP_055151
  Entrez Gene 59110 RatEntrez Gene 114230 MouseEntrez Gene 23746 Human
  UniProt ID Q9NZN9
• Background: Leber congenital amaurosis (LCA) accounts for at least 5% of all inherited retinal disease and is the most severe inherited retinopathy with the earliest age of onset. Individuals affected with LCA are diagnosed at birth or in the first few months of life with severely impaired vision or blindness, nystagmus and an abnormal or flat electroretinogram. The photoreceptor/pineal -expressed gene, AIPL1, encoding aryl-hydrocarbon interacting protein-like 1, was mapped within the LCA4 candidate region. The protein contains three tetratricopeptide motifs, consistent with nuclear transport or chaperone activity. AIPL1 mutations may cause approximately 20% of recessive LCA. [provided by RefSeq, Jul 2008]
• Synonyms: AIPL2; LCA4

Reference Data

• Protein Families: Druggable Genome