DPP10 Mouse Monoclonal Antibody [Clone ID: OTI1A5]

CAT#: CF503585

Carrier-free (BSA/glycerol-free) DPP10 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP10 (RC205435, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP10. Positive lysates LY412236 (100ug) and LC412236 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503585)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DPP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503585)

• 

  HEK293T cells transfected with either RC205435 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP10 antibody (TA503585), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP10(NP_065919) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.7 kDa
• Gene Name: Homo sapiens dipeptidyl peptidase like 10 (DPP10), transcript variant 1, mRNA.
• Database Link:
  NP_065919
  Entrez Gene 269109 MouseEntrez Gene 363972 RatEntrez Gene 57628 Human
  UniProt ID Q8N608
• Background: This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: DPL2; DPPY; DPRP-3; DPRP3

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane