CDKN3 Mouse Monoclonal Antibody [Clone ID: OTI5H1]

CAT#: CF503593

Carrier-free (BSA/glycerol-free) CDKN3 mouse monoclonal antibody, clone OTI5H1 (formerly 5H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (RC213080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3. Positive lysates LY417450 (100ug) and LC417450 (20ug) can be purchased separately from OriGene.

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  Anti-CDKN3 mouse monoclonal antibody (TA503593) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3 (RC213080).

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  HEK293T cells transfected with either RC213080 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody (TA503593), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5H1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDKN3(NP_005183) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.6 kDa
• Gene Name: Homo sapiens cyclin dependent kinase inhibitor 3 (CDKN3), transcript variant 1, mRNA.
• Database Link:
  NP_005183
  Entrez Gene 72391 MouseEntrez Gene 1033 Human
  UniProt ID Q16667
• Background: The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: CDI1; CIP2; KAP; KAP1

Reference Data

• Protein Families: Druggable Genome, Phosphatase