CNDP2 Mouse Monoclonal Antibody [Clone ID: OTI6D3]

CAT#: CF503631

Carrier-free (BSA/glycerol-free) CNDP2 mouse monoclonal antibody, clone OTI6D3 (formerly 6D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNDP2 (RC200120, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNDP2. Positive lysates LY413215 (100ug) and LC413215 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-CNDP2 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CNDP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503631)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNDP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503631)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CNDP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503631)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CNDP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503631)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CNDP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503631)

• Anti-CNDP2 mouse monoclonal antibody (TA503631) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CNDP2 (RC200120).

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Specifications

Product Data

• Clone Name: OTI6D3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CNDP2(NP_060705) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.7 kDa
• Gene Name: Homo sapiens carnosine dipeptidase 2 (CNDP2), transcript variant 1, mRNA.
• Database Link:
  NP_060705
  Entrez Gene 66054 MouseEntrez Gene 291394 RatEntrez Gene 55748 Human
  UniProt ID Q96KP4
• Background: CNDP2, also known as tissue carnosinase and peptidase A (EC 3.4.13.18), is a nonspecific dipeptidase rather than a selective carnosinase (Teufel et al., 2003 [PubMed 12473676]). [supplied by OMIM]
• Synonyms: CN2; CPGL; HEL-S-13; HsT2298; PEPA

Reference Data

• Protein Families: Protease