Nucleotide binding protein like (NUBPL) Mouse Monoclonal Antibody [Clone ID: OTI2C8]

CAT#: CF503725

Carrier-free (BSA/glycerol-free) NUBPL mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUBPL (RC204385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUBPL. Positive lysates LY410869 (100ug) and LC410869 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC204385 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUBPL antibody (TA503725), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2C8
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-250 of human NUBPL(NP_079428) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.9 kDa
• Gene Name: Homo sapiens nucleotide binding protein like (NUBPL), transcript variant 1, mRNA.
• Database Link:
  NP_079428
  Entrez Gene 76826 MouseEntrez Gene 299008 RatEntrez Gene 80224 Human
  UniProt ID Q8TB37
• Background: This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. The respiratory complex I consists of 45 subunits and 8 iron-sulfur (Fe/S) clusters. This protein is an Fe/S protein that plays a critical role in the assembly of respiratory complex I, likely by transferring Fe/S into the Fe/S-containing complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
• Synonyms: C14orf127; huInd1; IND1