TRK fused gene (TFG) Mouse Monoclonal Antibody [Clone ID: OTI2C3]

CAT#: CF503858

Carrier-free (BSA/glycerol-free) TFG mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFG (RC200293, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFG. Positive lysates LY423514 (100ug) and LC423514 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-TFG monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TFG-Knockout HeLa cells (KO, Cat# LC831921) were separated by SDS-PAGE and immunoblotted with anti-TFG monoclonal antibody TA503858 (1:200). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TFG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503858)

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Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TFG(NP_001007566) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.3 kDa
• Gene Name: Homo sapiens trafficking from ER to golgi regulator (TFG), transcript variant 2, mRNA.
• Database Link:
  NP_001007566
  Entrez Gene 360709 RatEntrez Gene 10342 Human
  UniProt ID Q92734
• Background: There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene. [provided by RefSeq]
• Synonyms: HMSNP; SPG57; TF6; TRKT3

Reference Data

• Protein Pathways: Pathways in cancer, Thyroid cancer