Pallidin (BLOC1S6) Mouse Monoclonal Antibody [Clone ID: OTI1H9]

CAT#: CF504279

Carrier-free (BSA/glycerol-free) PLDN mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (RC201514, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN. Positive lysates LY402203 (100ug) and LC402203 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PLDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504279)

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  HEK293T cells transfected with either RC201514 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody (TA504279), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1H9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PLDN(NP_036520) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.6 kDa
• Gene Name: Homo sapiens biogenesis of lysosomal organelles complex 1 subunit 6 (BLOC1S6), transcript variant 2, mRNA.
• Database Link:
  NP_036520
  Entrez Gene 18457 MouseEntrez Gene 317630 RatEntrez Gene 26258 Human
  UniProt ID Q9UL45
• Background: The protein encoded by this gene may play a role in intracellular vesicle trafficking. It interacts with Syntaxin 13 which mediates intracellular membrane fusion. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
• Synonyms: BLOS6; HPS9; PA; PALLID; PLDN