NPTN Mouse Monoclonal Antibody [Clone ID: OTI2D4]

CAT#: CF504330

Carrier-free (BSA/glycerol-free) NPTN mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPTN (RC210326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPTN. Positive lysates LY413759 (100ug) and LC413759 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NPTN monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and NPTN-Knockout HEK293T cells (KO, Cat# LC842402) were separated by SDS-PAGE and immunoblotted with anti-NPTN monoclonal antibody TA504330 (1:250). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NPTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504330)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NPTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504330)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NPTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504330)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NPTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504330)

• Anti-NPTN mouse monoclonal antibody (TA504330) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NPTN (RC210326).

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Specifications

Product Data

• Clone Name: OTI2D4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NPTN(NP_059429) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.7 kDa
• Gene Name: Homo sapiens neuroplastin (NPTN), transcript variant a, mRNA.
• Database Link:
  NP_059429
  Entrez Gene 20320 MouseEntrez Gene 56064 RatEntrez Gene 487637 DogEntrez Gene 699627 MonkeyEntrez Gene 27020 Human
  UniProt ID Q9Y639
• Background: This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2009]
• Synonyms: GP55; GP65; np55; np65; SDFR1; SDR1

Reference Data

• Protein Families: Druggable Genome, Transmembrane