ATIC Mouse Monoclonal Antibody [Clone ID: OTI1D2]

CAT#: CF504553

Carrier-free (BSA/glycerol-free) ATIC mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATIC (RC203490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATIC. Positive lysates LY418255 (100ug) and LC418255 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATIC monoclonal antibody.

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  HEK293T cells transfected with either RC203490 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATIC antibody (TA504553), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1D2
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATIC(NP_004035) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.4 kDa
• Gene Name: Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), mRNA.
• Database Link:
  NP_004035
  Entrez Gene 81643 RatEntrez Gene 108147 MouseEntrez Gene 488513 DogEntrez Gene 694819 MonkeyEntrez Gene 471 Human
  UniProt ID P31939
• Background: This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq]
• Synonyms: AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism