PGM3 Mouse Monoclonal Antibody [Clone ID: OTI1A11]

CAT#: CF504859

Carrier-free (BSA/glycerol-free) PGM3 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3. Positive lysates LY414446 (100ug) and LC414446 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504859)

• HEK293T cells transfected with either RC201522 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGM3 antibody (TA504859), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A11
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PGM3(NP_056414) produced in HKE293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.7 kDa
• Gene Name: Homo sapiens phosphoglucomutase 3 (PGM3), transcript variant 2, mRNA.
• Database Link:
  NP_056414
  Entrez Gene 109785 MouseEntrez Gene 363109 RatEntrez Gene 694464 MonkeyEntrez Gene 5238 Human
  UniProt ID O95394
• Background: This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]
• Synonyms: AGM1; IMD23; PAGM; PGM 3

Reference Data

• Protein Pathways: Amino sugar and nucleotide sugar metabolism