Cbl c (CBLC) Mouse Monoclonal Antibody [Clone ID: OTI3B4]

CAT#: CF505071

Carrier-free (BSA/glycerol-free) CBLC mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBLC (RC205130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBLC. Positive lysates LY402148 (100ug) and LC402148 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC205130 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CBLC antibody (TA505071), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3B4
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 244-474 of human CBLC(NP_036248) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.3 kDa
• Gene Name: Homo sapiens Cbl proto-oncogene C (CBLC), transcript variant 1, mRNA.
• Database Link:
  NP_036248
  Entrez Gene 23624 Human
  UniProt ID Q9ULV8
• Background: This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
• Synonyms: CBL-3; CBL-SL; RNF57

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis