EIF5A2 Mouse Monoclonal Antibody [Clone ID: OTI6F7]

CAT#: CF505147

Carrier-free (BSA/glycerol-free) EIF5A2 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2 (RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2. Positive lysates LY412495 (100ug) and LC412495 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505147)

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  HEK293T cells transfected with either RC206249 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EIF5A2 antibody (TA505147), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6F7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF5A2(NP_065123) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.6 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 5A2 (EIF5A2), mRNA.
• Database Link:
  NP_065123
  Entrez Gene 208691 MouseEntrez Gene 310261 RatEntrez Gene 56648 Human
  UniProt ID Q9GZV4
• Synonyms: EIF-5A2; eIF5AII