VTI1A Mouse Monoclonal Antibody [Clone ID: OTI1F4]

CAT#: CF505824

Carrier-free (BSA/glycerol-free) VTI1A mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VTI1A (RC213934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VTI1A. Positive lysates LY407945 (100ug) and LC407945 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VTI1A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VTI1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505824)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VTI1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505824)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VTI1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505824)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VTI1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505824)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VTI1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505824)

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Specifications

Product Data

• Clone Name: OTI1F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VTI1A(NP_660207) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: Homo sapiens vesicle transport through interaction with t-SNAREs 1A (VTI1A), transcript variant 2, mRNA.
• Database Link:
  NP_660207
  Entrez Gene 53611 MouseEntrez Gene 65277 RatEntrez Gene 100685447 DogEntrez Gene 696654 MonkeyEntrez Gene 143187 Human
  UniProt ID Q96AJ9
• Synonyms: MMDS3; MVti1; Vti1-rp2; VTI1RP2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport