Parvin alpha (PARVA) Mouse Monoclonal Antibody [Clone ID: OTI2B11]

CAT#: CF506000

Carrier-free (BSA/glycerol-free) PARVA mouse monoclonal antibody,clone OTI2B11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARVA (RC205086, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARVA. Positive lysates LY413202 (100ug) and LC413202 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PARVA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PARVA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506000)

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  Anti-PARVA mouse monoclonal antibody (TA506000) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PARVA (RC205086).

Specifications

Product Data

• Clone Name: OTI2B11
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PARVA(NP_060692) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.1 kDa
• Gene Name: Homo sapiens parvin alpha (PARVA), mRNA.
• Database Link:
  NP_060692
  Entrez Gene 57341 RatEntrez Gene 57342 MouseEntrez Gene 55742 Human
  UniProt ID Q9NVD7
• Background: This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq, Dec 2010]
• Synonyms: CH-ILKBP; MXRA2

Reference Data

• Protein Pathways: Focal adhesion