ACAA2 Mouse Monoclonal Antibody [Clone ID: OTI1B1]

CAT#: CF506129

Carrier-free (BSA/glycerol-free) ACAA2 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506129)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2 (RC201096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAA2. Positive lysates LY401843 (100ug) and LC401843 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• Anti-ACAA2 mouse monoclonal antibody (TA506129) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAA2 (RC201096).

• Immunofluorescent staining of HeLa cells using anti-ACAA2 mouse monoclonal antibody (TA506129).

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Specifications

Product Data

• Clone Name: OTI1B1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACAA2(NP_006102) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.7 kDa
• Gene Name: Homo sapiens acetyl-CoA acyltransferase 2 (ACAA2), mRNA.
• Database Link:
  NP_006102
  Entrez Gene 52538 MouseEntrez Gene 170465 RatEntrez Gene 490568 DogEntrez Gene 709350 MonkeyEntrez Gene 10449 Human
  UniProt ID P42765
• Background: The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq, Jul 2008]
• Synonyms: DSAEC

Reference Data

• Protein Pathways: Fatty acid elongation in mitochondria, Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation