IL10RA Mouse Monoclonal Antibody [Clone ID: OTI1D4]

CAT#: CF506934

Carrier-free (BSA/glycerol-free) IL10RA mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL10RA (RC207020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL10RA. Positive lysates LY400596 (100ug) and LC400596 (20ug) can be purchased separately from OriGene.

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  Anti-IL10RA mouse monoclonal antibody (TA506934) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL10RA (RC207020).

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  Immunofluorescent staining of HeLa cells using anti-IL10RA mouse monoclonal antibody (TA506934) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL10RA(NP_001549) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.8 kDa
• Gene Name: Homo sapiens interleukin 10 receptor subunit alpha (IL10RA), transcript variant 1, mRNA.
• Database Link:
  NP_001549
  Entrez Gene 3587 Human
  UniProt ID Q13651
• Background: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
• Synonyms: CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway