TXNDC5 Mouse Monoclonal Antibody [Clone ID: OTI1E2]

CAT#: CF507256

Carrier-free (glycerol/BSA-free) TXNDC5 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TXNDC5 (RC208568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TXNDC5.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TXNDC5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TXNDC5-Knockout 293T cells (KO, Cat# LC811412) were separated by SDS-PAGE and immunoblotted with anti-TXNDC5 monoclonal antibody TA507256, (1:100). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

Specifications

Product Data

• Clone Name: OTI1E2
• Applications: WB
• Recommended Dilution: WB 1:400~4000
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TXNDC5(NP_071368) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.5 kDa
• Gene Name: thioredoxin domain containing 5
• Database Link:
  NP_071368
  Entrez Gene 105245 MouseEntrez Gene 695213 MonkeyEntrez Gene 81567 Human
  UniProt ID Q8NBS9
• Background: This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq, Dec 2010]
• Synonyms: ERP46, Hcc-2, UNQ364, EndoPDI, MGC3178; thioredoxin domain containing 5

Reference Data

• Protein Families: Druggable Genome