ANKMY2 Mouse Monoclonal Antibody [Clone ID: OTI1F11]

CAT#: CF507305

Carrier-free (glycerol/BSA-free) ANKMY2 mouse monoclonal antibody, clone OTI1F11 (formerly 1F11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKMY2 (RC206770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKMY2. Positive lysates LY412559 (100ug) and LC412559 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANKMY2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ANKMY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507305)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANKMY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507305)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ANKMY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507305)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ANKMY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507305)

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Specifications

Product Data

• Clone Name: OTI1F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANKMY2(NP_064715) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: Homo sapiens ankyrin repeat and MYND domain containing 2 (ANKMY2), mRNA.
• Database Link:
  NP_064715
  Entrez Gene 217473 MouseEntrez Gene 314046 RatEntrez Gene 100856722 DogEntrez Gene 709414 MonkeyEntrez Gene 57037 Human
  UniProt ID Q8IV38
• Synonyms: ZMYND20