Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: OTI3B8]

CAT#: CF800649

Carrier-free (BSA/glycerol-free) MKI67 mouse monoclonal antibody, clone OTI3B8 (formerly 3B8)

Product Images

Western blot analysis of extracts (10ug) from 1 cell line by using anti-MKI67 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MKI67 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800649)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MKI67 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800649)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MKI67 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800649)

Specifications

Product Data

• Clone Name: OTI3B8
• Applications: IHC, WB
• Recommended Dilution: IHC 1:50~500, WB 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 1, mRNA.
• Database Link:
  NP_002408
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
• Synonyms: KIA; MIB-; MIB-1; PPP1R105

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS