DOCK8 Mouse Monoclonal Antibody [Clone ID: OTI7H2]

CAT#: CF801822

Carrier-free (BSA/glycerol-free) DOCK8 mouse monoclonal antibody, clone OTI7H2 (formerly 7H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOCK8 (RC222789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOCK8. Positive lysates LY404295 (100ug) and LC404295 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA801822)

Specifications

Product Data

• Clone Name: OTI7H2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 84-405 of human DOCK8 (NP_982272) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 238.3 kDa
• Gene Name: Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1, mRNA.
• Database Link:
  NP_982272
  Entrez Gene 76088 MouseEntrez Gene 499337 RatEntrez Gene 81704 Human
  UniProt ID Q8NF50
• Background: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
• Synonyms: HEL-205; MRD2; ZIR8