DOT1L Mouse Monoclonal Antibody [Clone ID: OTI1D8]

CAT#: CF802482

Carrier-free (BSA/glycerol-free) DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Positive lysates LY410109 (100ug) and LC410109 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802482)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802482)

Specifications

Product Data

• Clone Name: OTI1D8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-434 of human DOT1L (NP_115871) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens DOT1 like histone lysine methyltransferase (DOT1L), mRNA.
• Database Link:
  NP_115871
  Entrez Gene 84444 Human
  UniProt ID Q8TEK3
• Background: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
• Synonyms: DOT1; KMT4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation