beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: OTI1A9]

CAT#: CF802776

Carrier-free (BSA/glycerol-free) B2M mouse monoclonal antibody, clone OTI1A9 (formerly 1A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M. Positive lysates LY401311 (100ug) and LC401311 (20ug) can be purchased separately from OriGene.

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  Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# [TA803600]/TA803617/TA802776) at 5ug/ml and HRP conjugated B2M Detection mAb (Cat# [TA590171]) at 0.125ug/ml.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802776)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802776)

Specifications

Product Data

• Clone Name: OTI1A9
• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified native Human beta 2 Microglobulin protein
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.7 kDa
• Gene Name: Homo sapiens beta-2-microglobulin (B2M), mRNA.
• Database Link:
  NP_004039
  Entrez Gene 567 Human
  UniProt ID P61769
• Background: This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. [provided by RefSeq, Sep 2009]
• Synonyms: IMD43

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Antigen processing and presentation