ARL3 Mouse Monoclonal Antibody [Clone ID: OTI2G7]

CAT#: CF803088

Carrier-free (BSA/glycerol-free) ARL3 mouse monoclonal antibody, clone OTI2G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Positive lysates LY418068 (100ug) and LC418068 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803088)

Specifications

Product Data

• Clone Name: OTI2G7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-182 of human ARL3 (NP_004302) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.3 kDa
• Gene Name: Homo sapiens ADP ribosylation factor like GTPase 3 (ARL3), mRNA.
• Database Link:
  NP_004302
  Entrez Gene 403 Human
  UniProt ID P36405
• Background: ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]
• Synonyms: ARFL3

Reference Data

• Protein Families: Druggable Genome