RIP3 (RIPK3) Mouse Monoclonal Antibody [Clone ID: OTI1D8]

CAT#: CF803094

Carrier-free (BSA/glycerol-free) RIPK3 mouse monoclonal antibody,clone OTI1D8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3 (1:2000).

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  Western blot analysis of extracts (35ug) from A431 cell line by using anti-RIPK3 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1D8
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 233-518 of human RIPK3 (NP_006862) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens receptor interacting serine/threonine kinase 3 (RIPK3), mRNA.
• Database Link:
  NP_006862
  Entrez Gene 11035 Human
  UniProt ID Q9Y572
• Synonyms: RIP3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway