ITGB3BP Mouse Monoclonal Antibody [Clone ID: OTI4H1]

CAT#: CF803458

Carrier-free (BSA/glycerol-free) ITGB3BP mouse monoclonal antibody, clone OTI4H1 (formerly 4H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITGB3BP (RC200064, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITGB3BP. Positive lysates LY402304 (100ug) and LC402304 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4H1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ITGB3BP (NP_055103) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20 kDa
• Gene Name: Homo sapiens integrin subunit beta 3 binding protein (ITGB3BP), transcript variant 2, mRNA.
• Database Link:
  NP_055103
  Entrez Gene 23421 Human
  UniProt ID Q13352
• Background: This gene encodes a transcriptional coregulator that binds to and enhances the activity of members of the nuclear receptor families, thyroid hormone receptors and retinoid X receptors. This protein also acts as a corepressor of NF-kappaB-dependent signaling. This protein induces apoptosis in breast cancer cells through a caspase 2-mediated signaling pathway. This protein is also a component of the centromere-specific histone H3 variant nucleosome associated complex (CENP-NAC) and may be involved in mitotic progression by recruiting the histone H3 variant CENP-A to the centromere. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
• Synonyms: CENP-R; CENPR; HSU37139; NRIF3; TAP20

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors