TRIM27 Mouse Monoclonal Antibody [Clone ID: OTI2F9]

CAT#: CF804048

Carrier-free (BSA/glycerol-free) TRIM27 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM27 (RC202184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM27. Positive lysates LY401952 (100ug) and LC401952 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2F9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRIM27 (NP_006501) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.3 kDa
• Gene Name: Homo sapiens tripartite motif containing 27 (TRIM27), mRNA.
• Database Link:
  NP_006501
  Entrez Gene 19720 MouseEntrez Gene 291171 RatEntrez Gene 5987 Human
  UniProt ID P14373
• Background: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]
• Synonyms: RFP; RNF76

Reference Data

• Protein Families: Druggable Genome, Transcription Factors