PRAK (MAPKAPK5) Mouse Monoclonal Antibody [Clone ID: OTI4H4]

CAT#: CF804529

Carrier-free (BSA/glycerol-free) MAPKAPK5 mouse monoclonal antibody, clone OTI4H4 (formerly 4H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.

Specifications

Product Data

• Clone Name: OTI4H4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 197-471 of human MAPKAPK5 (NP_003659) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens MAPK activated protein kinase 5 (MAPKAPK5), transcript variant 1, mRNA.
• Database Link:
  NP_003659
  Entrez Gene 17165 MouseEntrez Gene 498183 RatEntrez Gene 8550 Human
  UniProt ID Q8IW41
• Synonyms: MAPKAP-K5; MK-5; MK5; PRAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway