Ocular development associated gene (GATAD1) Mouse Monoclonal Antibody [Clone ID: OTI1G10]

CAT#: CF804856

Carrier-free (glycerol/BSA-free) GATAD1 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATAD1 (RC202984, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATAD1. Positive lysates LY402849 (100ug) and LC402849 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 68-269 of human GATAD1 (NP_066990) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: Homo sapiens GATA zinc finger domain containing 1 (GATAD1), transcript variant 1, mRNA.
• Database Link:
  NP_066990
  Entrez Gene 67210 MouseEntrez Gene 500005 RatEntrez Gene 57798 Human
  UniProt ID Q8WUU5
• Background: The protein encoded by this gene contains a zinc finger at the N-terminus, and is thought to bind to a histone modification site that regulates gene expression. Mutations in this gene have been associated with autosomal recessive dilated cardiomyopathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]
• Synonyms: CMD2B; ODAG; RG083M05.2

Reference Data

• Protein Families: Transcription Factors