hHR23b (RAD23B) Mouse Monoclonal Antibody [Clone ID: OTI12C10]

CAT#: CF804868

Carrier-free (glycerol/BSA-free) RAD23B mouse monoclonal antibody, clone OTI12C10 (formerly 12C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Positive lysates LY401012 (100ug) and LC401012 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and RAD23B-Knockout Hela cells (KO, Cat# LC810196) were separated by SDS-PAGE and immunoblotted with anti-RAD23B monoclonal antibody TA804868. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Specifications

Product Data

• Clone Name: OTI12C10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-253 of human RAD23B (NP_002865) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: Homo sapiens RAD23 homolog B, nucleotide excision repair protein (RAD23B), transcript variant 1, mRNA.
• Database Link:
  NP_002865
  Entrez Gene 19359 MouseEntrez Gene 298012 RatEntrez Gene 5887 Human
  UniProt ID P54727
• Background: The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]
• Synonyms: HHR23B; HR23B; P58

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair