TGFBI Mouse Monoclonal Antibody [Clone ID: OTI9A11]

CAT#: CF805390

Carrier-free (BSA/glycerol-free) TGFBI mouse monoclonal antibody,clone OTI9A11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFBI (RC200411, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFBI (1:500). Positive lysates LY424775 (100ug) and LC424775 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HELA cell line by using anti-TGFBI monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from A549 cell line by using anti-TGFBI monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-TGFBI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805390) (1:500)

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Specifications

Product Data

• Clone Name: OTI9A11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 405-683 of human TGFBI (NP_000349) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.68 kDa
• Gene Name: Homo sapiens transforming growth factor beta induced (TGFBI), mRNA.
• Database Link:
  NP_000349
  Entrez Gene 21810 MouseEntrez Gene 116487 RatEntrez Gene 7045 Human
  UniProt ID Q15582
• Synonyms: BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane