NFS1 Mouse Monoclonal Antibody [Clone ID: OTI5D1]
CAT#: CF805820
Carrier-free (BSA/glycerol-free) NFS1 mouse monoclonal antibody, clone OTI5D1 (formerly 5D1)
Formulation: Standard
Other products for "NFS1"
Specifications
Product Data | |
Clone Name | OTI5D1 |
Applications | IHC, WB |
Recommended Dilution | WB 1:500, IHC 1:150 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment corresponding to amino acids 1-299 of human NFS1(NP_066923) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 50 kDa |
Gene Name | Homo sapiens NFS1 cysteine desulfurase (NFS1), transcript variant 1, mRNA. |
Database Link | |
Background | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov |
Synonyms | HUSSY-08; IscS; NIFS |
Reference Data | |
Protein Pathways | Thiamine metabolism |
Documents
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