Centrin 3 (CETN3) Mouse Monoclonal Antibody [Clone ID: OTI4G5]

CAT#: CF805822

Carrier-free (glycerol/BSA-free) CETN3 mouse monoclonal antibody, clone OTI4G5 (formerly 4G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CETN3 (RC202804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CETN3. Positive lysates LY418039 (100ug) and LC418039 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4G5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CETN3 (NP_004356) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.4 kDa
• Gene Name: Homo sapiens centrin 3 (CETN3), transcript variant 2, mRNA.
• Database Link:
  NP_004356
  Entrez Gene 1070 Human
  UniProt ID O15182
• Background: The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. [provided by RefSeq, Jul 2008]
• Synonyms: CDC31; CEN3

Reference Data

• Protein Families: Druggable Genome