MARK3 Mouse Monoclonal Antibody [Clone ID: OTI3F6]

CAT#: CF805880

Carrier-free (BSA/glycerol-free) MARK3 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Positive lysates LY400851 (100ug) and LC400851 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805880)

Specifications

Product Data

• Clone Name: OTI3F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 473-729 of human MARK3(NP_002367) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.3 kDa
• Gene Name: Homo sapiens microtubule affinity regulating kinase 3 (MARK3), transcript variant 3, mRNA.
• Database Link:
  NP_002367
  Entrez Gene 17169 MouseEntrez Gene 170577 RatEntrez Gene 4140 Human
  UniProt ID P27448
• Background: The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: CTAK1; KP78; Par-1a; PAR1A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase