MRRF Mouse Monoclonal Antibody [Clone ID: OTI5C11]

CAT#: CF806313

Carrier-free (BSA/glycerol-free) MRRF mouse monoclonal antibody, clone OTI5C11 (formerly 5C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRRF (RC203909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRRF (1:2000). Positive lysates LY408516 (100ug) and LC408516 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5C11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 56-262 of human MRRF(NP_620132) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.1 kDa
• Gene Name: Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.
• Database Link:
  NP_620132
  Entrez Gene 67871 MouseEntrez Gene 311903 RatEntrez Gene 92399 Human
  UniProt ID Q96E11
• Synonyms: MRFF; MTRRF; RRF

Reference Data

• Protein Families: Transmembrane