Angiopoietin 1 (ANGPT1) Mouse Monoclonal Antibody [Clone ID: OTI8C5]

CAT#: CF807191

Carrier-free (BSA/glycerol-free) ANGPT1 mouse monoclonal antibody, clone OTI8C5 (formerly 8C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPT1 (RC219398, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANGPT1 (1:500). Positive lysates LY400458 (100ug) and LC400458 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8C5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-320 of human ANGPT1(NP_001137) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.3 kDa
• Gene Name: Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA.
• Database Link:
  NP_001137
  Entrez Gene 11600 MouseEntrez Gene 89807 RatEntrez Gene 284 Human
  UniProt ID Q15389
• Background: Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2010]
• Synonyms: AGP1; AGPT; ANG1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein